How to measure emulsification index (E24)

In biotechnology field, this method is used to measure the emulsification ability of biosurfactant. Emulsification index (E24) is used to characterize the biosurfactant in emulsifying, generally, the hydrophobic phase in hydrophilic phase.

Following s are the emulsification index procedure based on the published journal titled “The production of rhamnolipid by a Pseudomonas aeruginosa strain isolated from a southern coastal zone in Brazil” by L.M. Prieto, et al.:

1. adding 2 ml of sample to the tube reaction
2. Adding oil to the same tube reaction on the same amount of sample
3. Mixing the solution with a vortex for 2 min
4. Leaving to stand for 24 h
5. The E24 is given as the percentage of the height of the emulsified layer (mm) divided by the total height of the liquid column (mm)
6. Remember to always use control positive and control negative in each measurement

Hopefully this procedure will help your experiment.

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