Tuesday, March 24, 2009

DNA Extraction Using Prepman Ultra

In my previous posting I’ve slightly given the bacterial DNA extraction method using Instagene matrix. In this particular posting, I want to give you the more simple method to extract DNA, which is using Prepman Ultra. According to the producer, Applied Biosystem, Prepman ultra is applicable to successfully preparing DNA template from bacteria, yeast, filamentous fungi, both from a plate or from tissue smears.

Here is the method:
  1. Picking out of 1 ose of the bacteria colony from the plate into 200 microliters of Prepman ultra reagent (placed in the sterile tube).
  2. Incubate the reagent+colony in 100 Celcius degree water-bath for 10 minutes.
  3. Cool the tube at the room temperature for 2 minutes.
  4. Centrifuge the tube at 10000 rpm for 2 minutes at 4 Celcius degree.
  5. Taking out the supernatant and leave the cell in the tube.
  6. Now you have 200 microliters of DNA template.
  7. You can transfer 5 microliters for the assay.

It is just as simple as that to get the DNA template, thanks to Applied Biosystem for this helpful kit.